AFMによるフォース測定

AFMは柔らかい生体サンプル(分子、細胞、組織など)から、ポリマーや硬い無機物まで、幅広い材料のメカニクスを測定できる強力なツールです。カンチレバーのばね定数に応じて、単体の分子をほどいたり(分子内)または2つの分子を引き離したり(分子間)するような、pNスケールの力を測定できます。
 

機能

  • 単分子のフォーススペクトロスコピー
  • pNの力の分解能で分子を引っ張る
  • ヤング率を測定するために、制御トリガー(フォースまたはディフレクション)をかけて材料を押す
  • フォース・クランピング
  • フォース・ランピング
  • 適切な溶液、温度で操作
  • ケミカルフォース顕微鏡
  • コロイドプローブAFM
 

一般的なアプリケーション

  • モジュラータンパク質をほどく;エネルギーランドスケープの測定・観察
  • DNA分子をほどく(B-S転移)
  • ポリマーの立体構造変化
  • 分子間の力の測定: リガンド‐レセプタ対、抗体‐抗原の結合、タンパク質‐ミネラル
  • 官能基と表面の間の特異性を測定
  • 凝着力を測定
  • 細胞(固定したもの、生きたもの、両方)、ポリマー、ゲルのメカニカル測定

Selected Publications

S. Assemi, J. Nalaskowski, and W. P. Johnson, "Direct force measurements between carboxylate-modified latex microspheres and glass using atomic force microscopy," Colloids Surf., A 286, 70-77 (2006). doi:10.1016/j.colsurfa.2006.03.024

A. Calò, D. Reguera, G. Oncins, M.-A. Persuy, G. Sanz, S. Lobasso, A. Corcelli, E. Pajot-Augy, and G. Gomila, "Force measurements on natural membrane nanovesicles reveal a composition-independent, high Young's modulus," Nanoscale 6, 2275 (2014). doi:10.1039/c3nr05107b

W.-J. Chung, K.-Y. Kwon, J. Song, and S.-W. Lee, "Evolutionary Screening of Collagen-like Peptides That Nucleate Hydroxyapatite Crystals," Langmuir 27, 7620-7628 (2011). doi:10.1021/la104757g

C. Dika, M. Ly-Chatain, G. Francius, J. Duval, and C. Gantzer, "Non-DLVO adhesion of F-specific RNA bacteriophages to abiotic surfaces: Importance of surface roughness, hydrophobic and electrostatic interactions," Colloids Surf., A 435, 178-187 (2013). doi:10.1016/j.colsurfa.2013.02.045

E. K. Dimitriadis, F. Horkay, J. Maresca, B. Kachar, and R. S. Chadwick, "Determination of Elastic Moduli of Thin Layers of Soft Material Using the Atomic Force Microscope," Biophys. J. 82, 2798-2810 (2002). doi:10.1016/s0006-3495(02)75620-8

T. Dugdale, R. Dagastine, A. Chiovitti, and R. Wetherbee, "Diatom Adhesive Mucilage Contains Distinct Supramolecular Assemblies of a Single Modular Protein," Biophys. J. 90, 2987-2993 (2006). doi:10.1529/biophysj.105.079129

R. W. Friddle, K. Battle, V. Trubetskoy, J. Tao, E. A. Salter, J. Moradian-Oldak, J. J. D. Yoreo, and A. Wierzbicki, "Single-Molecule Determination of the Face-Specific Adsorption of Amelogenin's C-Terminus on Hydroxyapatite," Angew. Chem. Int. Ed. 50, 7541-7545 (2011). doi:10.1002/anie.201100181

C. A. Grant, J. E. McKendry, and S. D. Evans, "Temperature dependent stiffness and visco-elastic behaviour of lipid coated microbubbles using atomic force microscopy," Soft Matter 8, 1321-1326 (2012). doi:10.1039/c1sm06578e

A. P. Gunning, A. R. Kirby, C. Fuell, C. Pin, L. E. Tailford, and N. Juge, "Mining the 'glycocode'--exploring the spatial distribution of glycans in gastrointestinal mucin using force spectroscopy," The FASEB Journal27, 2342-2354 (2013). doi:10.1096/fj.12-221416

T. Gutsmann, T. Hassenkam, J. A. Cutroni, and P. K. Hansma, "Sacrificial Bonds in Polymer Brushes from Rat Tail Tendon Functioning as Nanoscale Velcro," Biophys. J. 89, 536-542 (2005). doi:10.1529/biophysj.104.056747

Á. Karsai, M. S. Kellermayer, and S. P. Harris, "Mechanical Unfolding of Cardiac Myosin Binding Protein-C by Atomic Force Microscopy," Biophys. J. 101, 1968-1977 (2011). doi:10.1016/j.bpj.2011.08.030

M. S. Z. Kellermayer, L. Grama, A. Karsai, A. Nagy, A. Kahn, Z. L. Datki, and B. Penke, "Reversible Mechanical Unzipping of Amyloid β-Fibrils," J. Biol. Chem. 280, 8464-8470 (2004). doi:10.1074/jbc.m411556200

C. Lesoil, T. Nonaka, H. Sekiguchi, T. Osada, M. Miyata, R. Afrin, and A. Ikai, "Molecular shape and binding force of Mycoplasma mobile's leg protein Gli349 revealed by an AFM study," Biochem. Biophys. Res. Commun. 391, 1312-1317 (2010). doi:10.1016/j.bbrc.2009.12.023

A. Li, S. N. Ramakrishna, E. S. Kooij, R. M. Espinosa-Marzal, and N. D. Spencer, "Poly(acrylamide) films at the solvent-induced glass transition: adhesion, tribology, and the influence of crosslinking," Soft Matter8, 9092 (2012). doi:10.1039/c2sm26222c

B. H. Lower, L. Shi, R. Yongsunthon, T. C. Droubay, D. E. McCready, and S. K. Lower, "Specific Bonds between an Iron Oxide Surface and Outer Membrane Cytochromes MtrC and OmcA from Shewanella oneidensis MR-1," J. Bacteriol. 189, 4944-4952 (2007). doi:10.1128/jb.01518-06

J. A. C. Santos, L. M. Rebêlo, A. C. Araujo, E. B. Barros, and J. S. de Sousa, "Thickness-corrected model for nanoindentation of thin films with conical indenters," Soft Matter 8, 4441 (2012). doi:10.1039/c2sm07062f

P. Schwaderer, E. Funk, F. Achenbach, J. Weis, C. Bräuchle, and J. Michaelis, "Single-Molecule Measurement of the Strength of a Siloxane Bond," Langmuir 24, 1343-1349 (2008). doi:10.1021/la702352x

M. Seydou, Y. J. Dappe, S. Marsaudon, J.-P. Aimé, X. Bouju, and A.-M. Bonnot, "Atomic force microscope measurements and LCAO-S2 + vdW calculations of contact length between a carbon nanotube and a graphene surface," Phys. Rev. B 83, 045410 (2011). doi:10.1103/physrevb.83.045410

R. F. Tabor, C. Wu, F. Grieser, R. R. Dagastine, and D. Y. C. Chan, "Measurement of the Hydrophobic Force in a Soft Matter System," J. Phys. Chem. Lett. 4, 3872-3877 (2013). doi:10.1021/jz402068k

K. V. Vliet, G. Bao, and S. Suresh, "The biomechanics toolbox: experimental approaches for living cells and biomolecules," Acta Mater. 51, 5881-5905 (2003). doi:10.1016/j.actamat.2003.09.001